New microsatellite markers recognize differences in tandem repeats among four related Gastrodia species (Orchidaceae)

  • Ogaki Kenji
    Graduate School of Symbiotic Systems Science and Technology, Fukushima University
  • Suetsugu Kenji
    Department of Biology, Graduate School of Science, Kobe University
  • Kishikawa Keiju
    Graduate School of Symbiotic Systems Science and Technology, Fukushima University
  • Kyogoku Daisuke
    Graduate School of Agriculture, Kyoto University
  • Shutoh Kohtaroh
    Graduate School of Symbiotic Systems Science and Technology, Fukushima University The Hokkaido University Museum, Hokkaido University
  • Isagi Yuji
    Graduate School of Agriculture, Kyoto University
  • Kaneko Shingo
    Graduate School of Symbiotic Systems Science and Technology, Fukushima University

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タイトル別名
  • New microsatellite markers recognize differences in tandem repeats among four related <i>Gastrodia</i> species (Orchidaceae)

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<p>Gastrodia is the most species-rich genus among mycoheterotrophic plants, and is thus an essential taxon to understand the mechanism of species diversification in mycoheterotrophs. In this study, we developed microsatellite markers with high transferability for four Gastrodia species to examine genetic differentiation and similarity among species, populations and individuals. The 12 microsatellite markers developed from a G. fontinalis library showed high transferability for the ramets that identified G. nipponica, G. kuroshimensis and G. takeshimensis. In addition to the high transferability of these markers, we observed low allele variation within a sampled population of each species and allele differences among the four species. The 12 markers described here will be useful for investigating the genetic differences among and within the Gastrodia species, which evolved by a limitation of gene flow.</p>

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