カルボキシル基導入ポリリジンを用いた植物培養細胞の予備凍結法による超低温保存

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  • Cryopreservation of Plant Cultured Cells by Pre—Freezing Method Using Carboxylate Polylysine
  • カルボキシルキ ドウニュウ ポリリジン オ モチイタ ショクブツ バイヨウ サイボウ ノ ヨビ トウケツホウ ニ ヨル チョウテイオン ホゾン

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Suspension-cultured cells of Oryza sativa, were cryopreserved by the pre-freezing method. Dimethyl sulfoxide (DMSO), which is often used as a cryoprotectant, is cytotoxic. In this study, we examined the effect of carboxylate polylysine (PLL) as a cryoprotectant in replacement of DMSO. It was found that cryopreservation with 5% ethylene glycol and 3.5% PLL resulted in significantly higher survival rates than that with 5% DMSO and 10% glucose. Our results indicate that PLL is effective as a cryoprotectant for cryopreservation of cultured plant cells.

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