Structural Analysis of Cell Membranes by Small Convergence Angle HAADF-STEM Tomography

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Other Title
  • 小収束角HAADF-STEMトモグラフィによる細胞膜構造の解析
  • 小収束角HAADF-STEMトモグラフィによる細胞膜構造の解析--次世代のフリーズエッチトモグラフィの可能性に向けて
  • ショウシュウソクカク HAADF STEM トモグラフィ ニ ヨル サイボウマク コウゾウ ノ カイセキ ジセダイ ノ フリーズエッチトモグラフィ ノ カノウセイ ニ ムケテ
  • ～次世代のフリーズエッチトモグラフィの可能性に向けて～

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Description

<p>The rapidly-frozen and deeply-etched EM image allows us to find that the cytoplasmic surface of the plasma membrane has a large undulation covered with the networks of caveolae, clathrin-coated pits, and actin-based membrane skeletons. In this paper, we compared the three-dimensional reconstruction of the cellular freeze-replica containing of various focal point distances, between the conventional TEM tomography and the variable convergence-angle STEM tomography, by using of the electron microscope equipped with the three condenser-lens system containing Cs correctors. It was clearly found that the small convergence-angular (0.5mrad) HAADF-STEM tomography could make the best 3D reconstruction with little computational artifacts through the missing wedge during EM observation. This proposed freeze-etch tomography could be expected as a novel tool for the plasma membrane research.</p>

Journal

  • KENBIKYO

    KENBIKYO 44 (4), 257-261, 2009-12-30

    The Japanese Society of Microscopy

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