Isolation of a novel Co<sup>2+</sup>-resistant bacterium and the application of its siderophore in Co<sup>2+</sup> recovery from an aqueous solution
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- Shinozaki Yukiko
- Research Fellow of the Japan Society for the Promotion of Science National Institute for Agro-Environmental Sciences (NIAES)
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- Kitamoto Hiroko
- National Institute for Agro-Environmental Sciences (NIAES)
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- Sameshima-Yamashita Yuka
- Research Fellow of the Japan Society for the Promotion of Science National Institute for Agro-Environmental Sciences (NIAES)
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- Kinoshita Aya
- National Institute of Technology, Toyama College
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- Nakajima-Kambe Toshiaki
- Faculty of Life and Environmental Sciences, University of Tsukuba
抄録
<p>Siderophores are considered to have a good potential as decontamination agents owing to their metal-chelating abilities. In order to confirm whether siderophores can be used in the recovery of metal ions, a siderophore (or metallophore) exhibiting Co2+-chelating activity was screened to demonstrate its ability to recover Co2+ from an aqueous solution. A siderophore-producing bacterium, Pandoraea sp. HCo-4B, was identified from a screen of Co2+-resistant bacteria grown in an aerobic enrichment culture with a Co2+-supplemented medium. After incubation of the crude extracted siderophore in a Co2+-containing solution, the Co2+-siderophore complex was adsorbed on to a C18 column. The bound Co2+ was eluted from the column by the addition of 10 mM H2SO4. The recovered amount of Co2+ was proportional to the amount of the added siderophore. We observed that the siderophore identified in this study binds to Co2+ at a 1:1 ratio.</p>
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 65 (6), 273-276, 2019
公益財団法人 応用微生物学・分子細胞生物学研究奨励会