Characterization of Cellular Optoporation Using Nanosecond Laser and Metal Pattern for Massively Parallel Intranuclear Delivery

DOI

Bibliographic Information

Other Title
  • 超並列細胞核内デリバリに向けたナノ秒レーザと金属パターンによる細胞光穿孔法の解析

Abstract

<p>To advance intranuclear delivery of human cells, ns-pulsed laser was used for optoporation. Optical transfection techniques are less-invasive, clean, and efficient, hence optoporation is chosen. After growing cells on the substrate coated with a metallic layer of Titanium at specified micro-places, those are irradiated with laser using different energies. The goal is to optimize laser parameters for the same. Viability test and delivery rate is determined for characterization. Calcein AM and PI dye is used for viability test and FITC-dextran and PI dye is used to determine delivery rate. The study finds its major application in pharmaceutical industries, customization of medicines and drug discovery.</p>

Journal

Keywords

Details 詳細情報について

  • CRID
    1390565134829990400
  • NII Article ID
    130007800891
  • DOI
    10.11522/pscjspe.2019a.0_285
  • Text Lang
    en
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

Report a problem

Back to top