Effects of Forced Displacement Excitation on Cultured Osteoblasts

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  • 培養骨芽細胞に対する強制変位加振の影響

Abstract

<p>Mechanical stimulation is known to increase bone mass and density through the cells’ biochemical response. It was reported that proliferation and differentiation of cultured osteoblasts were promoted by mechanical vibration and the effects were depended on the frequency. However, the relationship between this result and the influence of sloshing of culture medium was not fully investigated. In this study, frequency-depended effect of the mechanical vibration was investigated under the condition where the sloshing is small enough by using a small diameter culture plate. Osteoblast-like cells were culture for non-vibration groups, 12.5 Hz, 0.5 G groups and 50 Hz, 0.5 G groups. The vibration groups were cultured under the vertical vibration for 24h/day. To observe the effect of proliferation, the number of cells was obtained. To observe the effect of differentiation, the area of mineralization was observed by alizarin red staining. As a result, the mechanical vibration of 12.5 Hz and 50 Hz both caused an increase in saturated cell density and a decrease in the time required for mineralization but there is no dtaistical difference between the effect of 12.5 Hz and 50 Hz. Comparison with previous studies, there is a possibility that frequency dependent effects of proliferation and differentiation may be attributed to sloshing.</p>

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