Inhibition of transcription factor OASIS attenuated kidney fibrosis

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  • Yamamoto Ayaha
    Laboratory of Clinical Science and Biomedicine, Grad. Sch. Pharm., Osaka Univ.
  • Obana Masanori
    Laboratory of Clinical Science and Biomedicine, Grad. Sch. Pharm., Osaka Univ.
  • Nakae Takafumi
    Laboratory of Clinical Science and Biomedicine, Grad. Sch. Pharm., Osaka Univ.
  • Miyake Yoshiaki
    Laboratory of Clinical Science and Biomedicine, Grad. Sch. Pharm., Osaka Univ.
  • Mitsuoka Sayuri
    Laboratory of Clinical Science and Biomedicine, Grad. Sch. Pharm., Osaka Univ.
  • Maeda Makiko
    Education and Research Project for Pharm.D, Grad. Sch. Pharm., Osaka Univ.
  • Imaizumi Kazunori
    Department of Biochemistry, Grad. Sch. Biomed. & Health Sci., Hiroshima Univ.
  • Matsumoto Kotaro
    Laboratory of Clinical Science and Biomedicine, Grad. Sch. Pharm., Osaka Univ.
  • Fujio Yasushi
    Laboratory of Clinical Science and Biomedicine, Grad. Sch. Pharm., Osaka Univ.

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Other Title
  • 転写因子OASISの阻害は腎線維化を抑制する

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<p>[Background] Since kidney fibrosis is a common pathway to many kidney diseases, prevention of kidney fibrosis could be a therapeutic strategy for kidney diseases. Previously, we reported that old astrocyte specifically induced substance (OASIS), a transcription factor, promotes proliferation of renal myofibroblasts, analyzed by in vitro assay. However, the pathophysiological significance of OASIS in kidney fibrosis in vivo remains to be elucidated.</p><p>[Methods/Results] C57BL/6J mice were subjected to unilateral ureteral obstruction (UUO) to cause kidney fibrosis, analyzed by Sirius red staining and hydroxyproline assay. AEBSF, an inhibitor of OASIS, suppressed kidney fibrosis at day 7 after UUO. Moreover, kidney fibrosis was ameliorated in OASIS KO mice compared with WT mice, accompanied by decreased proliferation of myofibroblasts. To explore the downstream of OASIS, DNA microarray was performed using myofibroblasts from OASIS KO mice. Interestingly, it was found that bone marrow stromal antigen 2 (BST2) was a candidate downstream gene of OASIS. Anti-BST2 antibody treatment attenuated UUO-induced kidney fibrosis.</p><p>[Conclusion] OASIS contributes to the develop of kidney fibrosis by promoting the proliferation of myofibroblasts, in part, through increased BST2 expression. OASIS could be a therapeutic target against kidney fibrosis.</p>

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