Endophytic actinomycetes associated with <i>Cinnamomum cassia</i> Presl in Hoa Binh province, Vietnam: Distribution, antimicrobial activity and, genetic features

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  • Vu Thi Hanh Nguyen
    Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST)
  • Nguyen Quang Huy
    Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST) Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST) University of Science and Technology of Hanoi (USTH), Vietnam Academy of Science and Technology (VAST)
  • Dinh Thi My Linh
    Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST)
  • Quach Ngoc Tung
    Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST)
  • Khieu Thi Nhan
    Department of Science, Technology and Environment, Ministry of Education and Training
  • Hoang Ha
    Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST)
  • Chu-Ky Son
    School of Biotechnology and Food Technology (SBFT), Hanoi University of Science and Technology (HUST)
  • Vu Thu Trang
    School of Biotechnology and Food Technology (SBFT), Hanoi University of Science and Technology (HUST)
  • Chu Hoang Ha
    Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST) Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST)
  • Lee Jusung
    The Center for Marine Natural Products and Drug Discovery, School of Earth and Environmental Sciences, College of Natural Sciences, Seoul National University NS-80
  • Kang Heonjoong
    The Center for Marine Natural Products and Drug Discovery, School of Earth and Environmental Sciences, College of Natural Sciences, Seoul National University NS-80 Research Institute of Oceanography, Seoul National University NS-80
  • Li Wen-Jun
    State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University
  • Phi Quyet-Tien
    Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST) Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST)

Description

<p>Endophytic microbes associated with medicinal plants are considered to be potential producers of various bioactive secondary metabolites. The present study investigated the distribution, antimicrobial activity and genetic features of endophytic actinomycetes isolated from the medicinal plant Cinnamomum cassia Presl collected in Hoa Binh province of northern Vietnam. Based on phenotypic characteristics, 111 actinomycetes were isolated from roots, stems and leaves of the host plants by using nine selective media. The isolated actinomycetes were mainly recovered from stems (n = 67; 60.4%), followed by roots (n = 29; 26.1%) and leaves (n = 15; 13.5%). The isolates were accordingly assigned into 5 color categories of aerial mycelium, of which gray is the most dominant (n = 42; 37.8%), followed by white (n = 33; 29.7%), yellow (n = 25; 22,5%), red (n = 8; 7.2%) and green (n = 3; 2.7%). Of the total endophytic actinomycetes tested, 38 strains (occupying 34.2%) showed antimicrobial activity against at least one of nine tested microbes and, among them, 26 actinomycetes (68.4%) revealed anthracycline-like antibiotics production. Analysis of 16S rRNA gene sequences deposited on GenBank (NCBI) of the antibiotic-producing actinomycetes identified 3 distinct genera, including Streptomyces, Microbacterium, and Nocardia, among which Streptomyces genus was the most dominant and represented 25 different species. Further genetic investigation of the antibiotic-producing actinomycetes found that 28 (73.7%) and 11 (28.9%) strains possessed genes encoding polyketide synthase (pks) and nonribosomal peptide synthetase (nrps), respectively. The findings in the present study highlighted endophytic actinomycetes from C. cassia Presl which possessed broad-spectrum bioactivities with the potential for applications in the agricultural and pharmaceutical sectors.</p>

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