Identification of Plant Fragments by Analyzing the Plastid <i>rpl16–rpl14</i> Linker Sequences

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  • Yamauchi Hiromasa
    Safety Science Institute, Suntory MONOZUKURI Expert Limited
  • Mukouzaka Yuri
    Safety Science Institute, Suntory MONOZUKURI Expert Limited
  • Harada Masami
    Quality Strategy Planning Department, MONOZUKURI Division, Suntory Holdings Limited

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  • 色素体<i>rpl16–rpl14</i>領域の塩基配列解析による飲料などに混入した植物片の識別
  • 色素体rpl16-rpl14領域の塩基配列解析による飲料などに混入した植物片の識別
  • シキソタイ rpl16-rpl14 リョウイキ ノ エンキ ハイレツ カイセキ ニ ヨル インリョウ ナド ニ コンニュウ シタ ショクブツヘン ノ シキベツ

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Abstract

<p>An identification method for testing contamination in products was assessed using various vegetables and fruits (70 types in total). DNA was extracted from plant fragments which are 1 to several millimeters long and the plastid rpl16–rpl14 linker sequence (approximately 550 base pairs) was amplified by PCR. The DNA nucleotide sequence was determined, and homology and SNP (single nucleotide polymorphism) analyses were carried out. Consequently, the test plants were difficult to distinguish between closely related species, but could be divided into 38 groups at the genus level or the species level. Although problems such as the accuracy of discrimination among some closely related plants and DNA stability under an acidic condition remain to be resolved, this method is considered to be expected to identify plant fragments mixed in products or raw materials.</p>

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