IMPROVEMENT OF UNEXPECTED ANTIBODY DETECTION USING 0.8% RBC SUSPENSION

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  • Watanabe Tomomi
    Department of Medical Technique, Nagoya University Hospital
  • Kato Chiaki
    Department of Medical Technique, Nagoya University Hospital
  • Endo Hiroko
    Department of Medical Technique, Nagoya University Hospital
  • Kawakami Moe
    Department of Medical Technique, Nagoya University Hospital
  • Nishida Kento
    Department of Medical Technique, Nagoya University Hospital
  • Matsushita Tadashi
    Department of Clinical Laboratory and Department of Transfusion Medicine, Nagoya University Hospital

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  • 0.8％赤血球浮遊液における不規則抗体の検出

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<p>Low-ionic-strength solution (LISS) is commonly used in column agglutination technology as potentiator in the detection of irregular antibody in pretransfusion testing.</p><p>In this study we evaluated the usefulness of 0.8% Ortho® Red Cell Diluent (RCD) with the ORTHO VISION® analyzer (Ortho Clinical Diagnostics Japan, Tokyo). Use of this test with an 0.8% RBC suspension (0.8% LISS-IAT) resulted in an increase in sensitivity for clinically significant antibodies and a decrease in the detection of clinically insignificant antibodies/nonspecific reactions compared to the test using LISS additive method with 4% RBC suspension (4% LISS-IAT). The 0.8% LISS-IAT detected newly produced antibodies, as early as PEG-IAT and earlier than LISS additive method. We speculate that the improvement in sensitivity with 0.8% LISS-IAT was due to a more appropriate ionic strength for antigen-antibody reaction, and efficient mixing in the reaction chamber.</p><p>We conclude that 0.8% LISS-IAT contributes to the safety of transfusion.</p>

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