インスリン分泌解析の新技術：生物発光法によるイメージングと定量解析

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  • Novel Technology for Studying Insulin Secretion: Imaging and Quantitative Analysis by a Bioluminescence Method
  • Symposium Review インスリン分泌解析の新技術 : 生物発光法によるイメージングと定量解析
  • Symposium Review インスリン ブンピ カイセキ ノ シン ギジュツ : セイブツ ハッコウホウ ニ ヨル イメージング ト テイリョウ カイセキ

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<p>We developed a method of video-rate bioluminescence imaging to visualize proteins secreted from living cells. A protein of interest was fused to Gaussia luciferase (GLase), and the luminescence signals of secreted GLase with coelenterazine (luciferin) were visualized at a video-rate of 30-500 ms/frame by using a water-cooled EM-CCD camera. We established a subclonal rat INS-1E cell line, named iGL cells, stably expressing the fusion protein of insulin and GLase (Insulin-GLase). By stimulation with high glucose, 3D-cultured iGL cells showed synchronized oscillatory secretion of insulin for over 1 h, as similarly observed in an isolated rat pancreatic islet. In 2D-cultured iGL cells, the luminescence images indicated that synchronized insulin secretion was localized in intercellular spaces between cells. Further, the relative amount of insulin secretion from iGL cells was easily determined with a luminometer, and we demonstrated that cell-cell interaction of beta cells is fundamental to increase glucose-stimulated insulin secretion by synchronization. Thus, iGL cells would be valuable for studying oscillatory insulin secretion and evaluating anti-diabetic drugs. Our bioluminescence imaging method with GLase could be generally used for investigating protein secretion in 2D and 3D cell culture systems.</p>

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  • 薬学雑誌

    薬学雑誌 140 (8), 969-977, 2020-08-01

    公益社団法人 日本薬学会

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