日本薬局方・定量用試薬の規格化を目的とした定量NMRを用いた吸湿性化合物の絶対純度の測定（Part 1）

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書誌事項

タイトル別名
  • Determination of Absolute Purities of Hygroscopic Substances by Quantitative NMR Analysis for the Standardization of Quantitative Reagents in the Japanese Pharmacopoeia (Part 1)
  • ニホン ヤッキョクカタ ・ テイリョウヨウ シヤク ノ キカクカ オ モクテキ ト シタ テイリョウ NMR オ モチイタ キュウシツセイ カゴウブツ ノ ゼッタイ ジュンド ノ ソクテイ(Part 1)

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説明

<p>Quantitative NMR (qNMR) has been developed as an absolute quantitation method to determine the purity or content of organic compounds including marker compounds in crude drugs. The “qNMR test” has been introduced into the crude-drug section of the Japanese Pharmacopoeia (JP) for determining the purity of reagents used for the assay in the JP. In Supplement II to the JP 17th edition published in June 2019, fifteen compounds adopted qNMR test were listed as the reagents for the assay. To establish the “qNMR test” in the crude drug section of the JP, there were several problems to be solved. Previously, we reported that the handling impurity signals from reference substances and targeted marker compounds, chemical shifts of reference substances, and peak unity of signals of targeted marker compounds are important factors to conduct qNMR measurements with intended accuracy. In this study, we investigated that the hygroscopicity of reagents could cause the changes in the compounds' purity depending on increasing their water content. Twenty-one standard products used for the crude-drug test in JP were examined by water sorption-desorption analysis, and ginsenosides and saikosaponins were found to be hygroscopic. To prepare a sample solution of saikosaponin b2 for qNMR analysis, samples need to be maintained for 18 h at 25°C and 76% relative humidity; further, samples need to be weighed at the same humidity for the qNMR analysis.</p>

収録刊行物

  • 薬学雑誌

    薬学雑誌 140 (8), 1063-1069, 2020-08-01

    公益社団法人 日本薬学会

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