Conditional kisspeptin neuron-specific Kiss1 knockout with newly generated Kiss1-floxed and Kiss1-Cre mice replicates a hypogonadal phenotype of global Kiss1 knockout mice
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- IKEGAMI Kana
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- GOTO Teppei
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
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- NAKAMURA Sho
- Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- WATANABE Youki
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- SUGIMOTO Arisa
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- MAJARUNE Sutisa
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- HORIHATA Kei
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- NAGAE Mayuko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- TOMIKAWA Junko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- IMAMURA Takuya
- Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima 739-8526, Japan
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- SANBO Makoto
- Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
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- HIRABAYASHI Masumi
- Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
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- INOUE Naoko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- MAEDA Kei-ichiro
- Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- TSUKAMURA Hiroko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- UENOYAMA Yoshihisa
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
書誌事項
- タイトル別名
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- Conditional kisspeptin neuron-specific <i>Kiss1</i> knockout with newly generated <i>Kiss1</i>-floxed and <i>Kiss1</i>-Cre mice replicates a hypogonadal phenotype of global <i>Kiss1</i> knockout mice
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説明
<p> The present study aimed to evaluate whether novel conditional kisspeptin neuron-specific Kiss1 knockout (KO) mice utilizing the Cre-loxP system could recapitulate the infertility of global Kiss1 KO models, thereby providing further evidence for the fundamental role of hypothalamic kisspeptin neurons in regulating mammalian reproduction. We generated Kiss1-floxed mice and hypothalamic kisspeptin neuron-specific Cre-expressing transgenic mice and then crossed these two lines. The conditional Kiss1 KO mice showed pubertal failure along with a suppression of gonadotropin secretion and ovarian atrophy. These results indicate that newly-created hypothalamic Kiss1 KO mice obtained by the Cre-loxP system recapitulated the infertility of global Kiss1 KO models, suggesting that hypothalamic kisspeptin, but not peripheral kisspeptin, is critical for reproduction. Importantly, these Kiss1-floxed mice are now available and will be a valuable tool for detailed analyses of roles of each population of kisspeptin neurons in the brain and peripheral kisspeptin-producing cells by the spatiotemporal-specific manipulation of Cre expression.</p>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 66 (4), 359-367, 2020
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390566775159848448
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- NII論文ID
- 130007888736
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 030626395
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- PubMed
- 32307336
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可