Suppressive effects of black soybean seed coat extract (Chrono-Care <sup>® </sup>) supplementation on <i>N</i>-methyl-<i>N</i>-nitrosourea-induced retinal degen eration in rats

DOI
  • Tatsuda Hikari
    Department of Food Sciences and Nutrition, Mukogawa Womenʼs University
  • Koyama Chihiro
    Administration Food Sciences and Nutrition Major, Graduate School of Human Environmental Sciences, Mukogawa Women's University
  • Takenouchi Akiko
    Department of Food Sciences and Nutrition, Mukogawa Womenʼs University
  • Yoshioka Masahiro
    Department of Nutritional Science, Koshien University
  • Akagi Ryota
    R&D Laboratories, Fujicco Co., Ltd.
  • Maruo Toshinari
    R&D Laboratories, Fujicco Co., Ltd.
  • Suzuki Toshio
    R&D Laboratories, Fujicco Co., Ltd.
  • Yuri Takashi
    Department of Nutritional Science, Koshien University
  • Yoshizawa Katsuhiko
    Department of Food Sciences and Nutrition, Mukogawa Womenʼs University Administration Food Sciences and Nutrition Major, Graduate School of Human Environmental Sciences, Mukogawa Women's University

Bibliographic Information

Other Title
  • 黒大豆種皮抽出物（クロノケア® ）を用いた <i>N</i>-メチル-<i>N</i>- ニトロソ尿素誘発ラット網膜変性症モデルにおける病態抑制効果

Description

<p>Retinitis pigmentosa (RP) is an ophthalmic disease with no fundamental cure that eventually leads to blindness. Since RP is associated with apoptosis of photoreceptor cells caused by oxidative stress, suppression of oxidative stress is expected to be effective in ameliorating RP. Since black soybean seed coat extract has high antioxidant activity, we used Chrono-Care® (Chrono-Care ® SP60, Fujicco Co., Ltd.) that contains black soybean polyphenol to investigate suppressive effects on N-methyl-N-nitorsourea (MNU) -induced retinal degeneration in rats. The antioxidant effects of 1, 2, and 4% Chrono-Care® water were measured by an OXY-adsorbent test. Female SD rats at 7 weeks of age were given a single i.p. injection of 50 mg/kg MNU and 0.2 or 2% Chrono Care ® -containing diets were freely fed from 4 weeks of age until the day of necropsy. Eyes were collected 48 hrs after MNU injection and the photoreceptor cell ratio was calculated. Using the same animal model, rats were freely fed 2% diet on the same schedule. Eyes were collected 24 hrs and 7 days after MNU injection for TUNEL and other immunostaining. In the results, concentration-dependent increase of antioxidant activity was observed. In the 2% diet combined group, retinal photoreceptor cell ratio was higher than in the MNU treated group. In the 2% diet combined group, TUNEL and anti-thymidine glycol immunostaining signals were lower than in the MNU treated group 24 hrs after MNU injection. Rhodopsin positive immunostaining was extensive in outer retina 7 days after MNU injection. In conclusion, suppressive effects of Chrono-Care ® SP60 on MNU induced retinal degeneration was demonstrated. The mode of action is potentially related to the suppression of oxidative stress in the retina. It may possibly be effective in suppressing of human RP.</p>

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Keywords

Details 詳細情報について

  • CRID
    1390567172569256832
  • NII Article ID
    130007899891
  • DOI
    10.32153/ffr.ffr191212-2
  • ISSN
    24343048
    24323357
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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