Surveillance of Shiga toxin-producing <i>Escherichia coli</i> and <i>Campylobacter</i> spp. in wild Japanese deer (<i>Cervus nippon</i>) and boar (<i>Sus scrofa</i>)

  • TOMINO Yoshiyuki
    Laboratory of Veterinary Public Health, Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan
  • ANDOH Masako
    Laboratory of Pathological and Preventive Veterinary Science, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan
  • HORIUCHI Yuta
    Laboratory of Veterinary Public Health, Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan
  • SHIN Jiye
    Laboratory of Veterinary Public Health, Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan
  • AI Ryunosuke
    Laboratory of Pathological and Preventive Veterinary Science, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan
  • NAKAMURA Takaki
    Laboratory of Pathological and Preventive Veterinary Science, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan
  • TODA Mizuki
    Laboratory of Pathological and Preventive Veterinary Science, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan
  • YONEMITSU Kenzo
    Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yamaguchi, Yamaguchi 753-8515, Japan
  • TAKANO Ai
    Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yamaguchi, Yamaguchi 753-8515, Japan
  • SHIMODA Hiroshi
    Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yamaguchi, Yamaguchi 753-8515, Japan
  • MAEDA Ken
    Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yamaguchi, Yamaguchi 753-8515, Japan
  • KODERA Yuuji
    Center for Weed and Wildlife Management, Utsunomiya University, 350 Mine-machi, Utsunomiya, Tochigi 321-8505, Japan
  • OSHIMA Ichiro
    Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan
  • TAKAYAMA Koji
    Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan
  • INADOME Takayasu
    General Incorporated Foundation Kagoshima Environmental Research and Service, 1-1-5 Nanatsujima, Kagoshima, Kagoshima 891-0132, Japan
  • SHIOYA Katsunori
    General Incorporated Foundation Kagoshima Environmental Research and Service, 1-1-5 Nanatsujima, Kagoshima, Kagoshima 891-0132, Japan
  • FUKAZAWA Motoki
    Tamagawa University Farm Kushi, Kagoshima, 880 Bonotsucho Kushi, Misamisatuma, Kagoshima 898-0211, Japan
  • ISHIHARA Kanako
    Laboratory of Veterinary Public Health, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan
  • CHUMA Takehisa
    Laboratory of Veterinary Public Health, Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan Laboratory of Pathological and Preventive Veterinary Science, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan

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  • Surveillance of Shiga toxin-producing Escherichia coli and Campylobacter spp. in wild Japanese deer (Cervus nippon) and boar (Sus scrofa)

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<p>Increasing game meat consumption in Japan requires the dissemination of safety information regarding the presence of human pathogens in game animals. Health information regarding the suitability of these animals as a meat source is not widely available. In this study, we aimed to evaluate the safety of game meat and detect potential human pathogens in wild deer (Cervus nippon) and boar (Sus scrofa) in Japan. Fecal samples from 305 wild deer and 248 boars of Yamaguchi, Kagoshima, and Tochigi prefectures collected monthly for 2 years were examined for the prevalence of Shiga toxin-producing Escherichia coli (STEC) and Campylobacter spp. STEC was isolated from 51 deer consistently throughout the year and from three boars; O-antigen genotype O146, the expression of stx2b, and eaeA absence (n=33) were the major characteristics of our STEC isolates. Other serotypes included the medically important O157, stx2b or stx2c, and eaeA-positive (n=4) and O26, stx1a, and eaeA-positive strains (n=1). Campylobacter spp. were isolated from 17 deer and 31 boars. Campylobacter hyointestinalis was the most common species isolated from 17 deer and 25 boars, whereas Campylobacter lanienae and Campylobacter coli were isolated from three and two boars, respectively. Seasonal trends for the isolation of these bacteria were not significant. This study demonstrates that wild game animals carry human pathogens; therefore, detailed knowledge of the safe handling of game meat is needed to prevent foodborne infections.</p>

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