Definitive methods for puncture examination (proposed)—cell counting method, cell classification method, and reporting method: Pleural effusion, ascites fluid, pericardial effusion, synovial fluid, continuous ambulatory peritoneal dialysis (CAPD)

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  • HOSHINA Hizuru
    Laboratory Medicine, Suwa Central Hospital The Working Group for the Standardization of Puncture Fluid Established in Japanese Association of Medical Technologists
  • HABARA Toshiyuki
    Laboratory Medicine, Chugoku Central Hospital The Working Group for the Standardization of Puncture Fluid Established in Japanese Association of Medical Technologists
  • OZEKI Noriyuki
    Clinical Laboratory Department, Dokkyo Medical University Saitama Medical Center The Working Group for the Standardization of Puncture Fluid Established in Japanese Association of Medical Technologists
  • UCHIDA Kazuho
    Clinical Laboratory Department, Toyohashi Municipal Hospital The Working Group for the Standardization of Puncture Fluid Established in Japanese Association of Medical Technologists
  • OKADA Shigeharu
    Department of Clinical Laboratory, Saitama Cancer Center The Working Group for the Standardization of Puncture Fluid Established in Japanese Association of Medical Technologists
  • OZAWA Masaru
    Department of Clinical Testing, Kyoto College of Health and Hygiene The Working Group for the Standardization of Puncture Fluid Established in Japanese Association of Medical Technologists

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  • 穿刺液検体の検査法―細胞数と細胞分類を中心に―～胸水，腹水，心嚢水，関節液，CAPD排液～

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<p>A working group for the standardization of puncture fluid established by the Japanese Association of Medical Technologists has compiled the methods of cytometry, cytotaxonomy, and reporting for the puncture fluid test. First, cells were counted, except red blood cells. Next, we employed the Samson classification method and Giemsa staining for the cytotaxonomic classification of three cell types. The Samson classification method classified cells into polymorphonuclear cells, lymphocytes, and other cells. Giemsa staining classified cells into neutrophils, lymphocytes, and other cells, and the percentage of each type of cell was shown. If the details of other cells were known, they would be recorded as supplemental information. The reports based on the results of these classification methods may indicate acute inflammation, chronic inflammation, or leaky pathology. They were also useful for primary screening and for determining the next tests, such as microbiology tests and cytology.</p>

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