Locus-specific analysis of DNA methylation patterns in cloned and in vitro fertilized porcine embryos

DOI Web Site Web Site PubMed 参考文献42件
  • XU Weihua
    Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, College of Life Sciences, Longyan University, Longyan 364012, P. R. China Provincial Key Laboratory for Developmental Biology and Neurosciences, College of Life Sciences, Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, P. R. China
  • LI Hongyi
    Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, College of Life Sciences, Longyan University, Longyan 364012, P. R. China
  • ZHANG Mao
    Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, College of Life Sciences, Longyan University, Longyan 364012, P. R. China
  • SHI Junsong
    Guangdong Provincial Wen’s Research Institute, Yunfu 527400, P. R. China
  • WANG Zhengchao
    Provincial Key Laboratory for Developmental Biology and Neurosciences, College of Life Sciences, Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, P. R. China

書誌事項

タイトル別名
  • Locus-specific analysis of DNA methylation patterns in cloned and <i>in vitro</i> fertilized porcine embryos

この論文をさがす

説明

<p> Porcine somatic cell nuclear transfer (SCNT) is currently inefficient, as 1–3.95% of reconstructed embryos survive to term; inadequate or erroneous epigenetic reprogramming of the specialized donor somatic nucleus could be a primary reason. Therefore, a locus-specific analysis of DNA methylation dynamics in embryogenesis and the DNA methylation status of gametes and donor cells used for SCNT were conducted in the following developmentally important gene loci: POU5F1, NANOG, SOX2, H19, IGF2, IGF2R, XIST; and the retrotransposon LINE-1. There were significant epigenetic differences between the gametes and the somatic donor cells. Three gamete-specific differentially methylated regions (DMRs) in POU5F1, XIST, and LINE-1 were identified. A delayed demethylation process at POU5F1 and LINE-1 loci occurred after three successive cleavages, compared to the in vitro fertilized (IVF) embryos. Although cloned embryos could undergo de-methylation and re-methylation dynamics at the DMRs of imprinted genes (H19, IGF2R, and XIST), the re-methylation process was compromised, unlike in fertilized embryos. LINE-1 loci are widely dispersed across the whole genome, and LINE-1 DMR might be a potential porcine nuclear reprogramming epi-marker. Data from observations in our present and previous studies, and two published articles were pooled to produce a schematic diagram of locus-specific, DNA methylation dynamics of cloned and IVF embryos during porcine early embryogenesis. This also indicated aberrant DNA methylation reprogramming events, including inadequate DNA demethylation and insufficient re-methylation in cloned embryos. Further research should focus on mechanisms underlying demethylation during the early cleavage of embryos and de novo DNA methylation at the blastocyst stage.</p>

収録刊行物

参考文献 (42)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ