Alteration of Mouse Nasal Airway Surface Mucociliary Transit by Airway Rehydrating Agents

DOI
  • Donnelley Martin
    Respiratory & Sleep Medicine, Women’s and Children’s Hospital Robinson Research Institute, University of Adelaide School of Medicine, University of Adelaide
  • Morgan Kaye
    School of Physics, Monash University
  • Farrow Nigel
    Respiratory & Sleep Medicine, Women’s and Children’s Hospital Robinson Research Institute, University of Adelaide School of Medicine, University of Adelaide
  • Carnibella Richard
    Mechanical and Aerospace Engineering, Monash University
  • Murrie Rhiannon
    Mechanical and Aerospace Engineering, Monash University
  • Fouras Andreas
    Mechanical and Aerospace Engineering, Monash University
  • Parsons David
    Respiratory & Sleep Medicine, Women’s and Children’s Hospital Robinson Research Institute, University of Adelaide School of Medicine, University of Adelaide

Description

Cystic fibrosis (CF) is a genetic disorder that compromises the ability of the mucociliary transit (MCT) system to clear debris and pathogens from the airways. To directly assess airway health and the effects of treatments we have developed a synchrotron X-ray microscopy method that non-invasively measures the local rate and patterns of MCT behaviour. The aim of this experiment was to determine if our non-invasive local airway health assessment method could identify changes in nasal MCT rate following clinical treatments known to alter MCT.<br> Experiments were performed on the BL20XU beamline at the SPring-8 Synchrotron in Japan. Mice were anaesthetized, a small quantity of lead sulphide particles were delivered to the nose, and images of the nasal airways were acquired. The nasal airways were treated with hypertonic saline or mannitol to increase surface hydration and change MCT. Custom software was used to locate and track the motion of the lead particles throughout the image sequences, and to calculate individual and bulk MCT rates.<br> MCT rates increased following both treatments, but due to high variability there were no statistically significant differences in MCT rate between treatments. However, in future studies we hope that the improved sensitivity provided by this technique will allow us to identify useful CF lung disease-modifying therapies.

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Details 詳細情報について

  • CRID
    1390568456352565248
  • NII Article ID
    130007969312
  • DOI
    10.18957/rr.5.1.15
  • ISSN
    21876886
  • Text Lang
    en
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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