Odontogenic differentiation of inflamed dental pulp stem cells (IDPSCs) on polycaprolactone (PCL) nanofiber blended with hydroxyapatite
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- GOPINATH Vellore Kannan
- College of Dental Medicine, University of Sharjah, University City
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- SOUMYA Sheela
- Sharjah Institute for Medical Research, University of Sharjah, University City
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- CHAKRAPANI V. YOGESHWAR
- Medical Material Laboratory, Indian Institute of Technology Madras
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- KUMAR T.S. SAMPATH
- Medical Material Laboratory, Indian Institute of Technology Madras
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<p>The objective of this study was to develop electrospun polycaprolactone (PCL) membranes blended with hydroxyapatite (HA) and evaluate its potential in differentiating inflamed dental pulp stem/progenitor cells (IDPSCs) into odontoblasts. Electrospun nanofibrous membrane consisting of PCL blended with 10 wt% and 15 wt% of HA were fabricated and the characterization was done by Scanning electron microscopy (SEM), Fourier- transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and contact angle analysis. Cytocompatibility, cell adhesion and odontogenic differentiation ability of the membranes were assessed by MTT, Live/Dead, SEM/DAPI and qPCR studies. The mineral deposition ability of the membranes with IDPSCs was estimated by SEM-EDS. The SEM analysis revealed a nanofibrous texture with an average fiber diameter of 140 nm for PCL, 220 nm for PCL10%HA and 250 nm for PCL15%HA. Among the membranes tested, PCL10%HA favored positive cell attachments, upregulated expression of DSPP and ALP gene and higher Ca/P ratio compared to PCL and PCL15%HA.</p>
収録刊行物
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- Dental Materials Journal
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Dental Materials Journal 40 (2), 312-321, 2021-03-25
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