Nervous Necrosis Virus (NNV) -Susceptibility and -Productivity of Newly Established Cell Lines Derived from Sevenband Grouper <i>Hyporthodus septemfasciatus</i>

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  • Nervous Necrosis Virus (NNV) -Susceptibility and -Productivity of Newly Established Cell Lines Derived from Sevenband Grouper Hyporthodus septemfasciatus

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<p>Multiple cell lines permissive to nervous necrosis virus (NNV) infection may be necessary for identifying receptor binding sites of viral surface protrusions. ​We have established new cell lines, SeGE-12, SeGE-22, SeGF and SeGB, derived from eyed-egg embryos, fin and brain of sevenband grouper. ​The optimum growth temperatures of these cell lines were all around 30°C. ​The growth temperature range for SeGE-12 and SeGE-22 was 25°C–32°C, whereas for SeGF and SeGB it was 15°C–32°C. ​These cell lines exhibited no cytopathic effect (CPE) due to NNV infection, but released extracellular NNV at 102.8 to 104.7 TCID50/mL after 14 days incubation.</p>

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  • Fish Pathology

    Fish Pathology 56 (1), 22-25, 2021-03-15

    The Japanese Society of Fish Pathology

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