Laboratory reconstruction and nearby sulfide dynamics of a model benthic boundary layer containing sediment-mixed biofilm

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  • ISHIBASHI Norihisa
    Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology
  • SATOH Hiroo
  • KANDA Jota
    Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology

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Other Title
  • セディメント・ミックスバイオフィルムを有する底部境界モデル培養系の確立と硫化物動態

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Abstract

Sediment-mixed biofilms of microorganisms embedded within an extracellular polymeric substance were reconstructed using laboratory models of seawater-benthic boundaries, and the sulfide dynamics in the boundary zone were examined. Biofilm-covered sediment was obtained from the field and cultured in a beaker of seawater with a granular formula for fish larvae and powdered foraminiferal limestone. In the standing culture, a floating biofilm formed on the liquid surface, and a microbiota capable of mixed biofilm formation was obtained. The mixed biofilm was cultured in a fluidization-free aerobic state, again with nutritional granules and powdered foraminiferal limestone. This culture formed a sediment-mixed biofilm on the bottom of the beaker. Furthermore, a seawater-benthic boundary model was developed on a hydrated substratum with sediment biofilm, using a nylon mesh as the model bottom layer in the culture system. When the floating biofilm was disrupted and then dispersed on the nylon mesh, detectable biofilms formed on the surface of the model bottom. As nutrients were utilized by the microbes, sulfides accumulated beneath the model bottom. Subsequently, the sulfides passed through the model bottom and became detectable in the seawater just above the sediment-mixed biofilm, where a white-turbid layer formed. As revealed by denaturing gradient gel electrophoresis, the layer contained populations of several bacterial species.

Journal

  • La mer

    La mer 55 (3-4), 73-88, 2017

    The Japanese-French Oceanographic Society

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