The cardiorespiratory responses to inhalation and pentobarbital anesthesia in the mouse
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- Han Chong
- Department of Pharmacology, Hirosaki University Graduates School of Medicine
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- Ogata Yoshiki
- Department of Pharmacology, Hirosaki University Graduates School of Medicine
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- Niwa Hidetoshi
- Department of Anesthesiology, Hirosaki University Graduates School of Medicine
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- Kushikata Tetsuya
- Department of Anesthesiology, Hirosaki University Graduates School of Medicine
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- Watanabe Hiroyuki
- Department of Internal Medicine Division of Cardiovascular and Respiratory Medicine, Akita University School of Medicine
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- Imaizumi Tadaatsu
- Department of Vascular Biology, Institute of Brain Science, Hirosaki University Graduates School of Medicine
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- Hirota Kazuyoshi
- Department of Anesthesiology, Hirosaki University Graduates School of Medicine
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- Ono Kyouichi
- Department of Cellphysiology, Akita University School of Medicine
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- Ohba Takayoshi
- Department of Cellphysiology, Akita University School of Medicine
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- Murakami Manabu
- Department of Pharmacology, Hirosaki University Graduates School of Medicine
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抄録
Transgenic mice experiments have become increasingly popular to research human inherited disease. However, a number of Japanese researchers have difficulty with the selection of anesthesia, after the classification of ketamine, probably the most used anesthesia, as a narcotic drug in 2006. Therefore, we compared the effects of inhalation anesthesia (2% of isoflurane, sevoflurane and enflurane) and intraperitoneal pentobarbital anesthesia (50mg/kg) on the electrocardiogram (ECG) and blood oxygen saturation (SPO₂) of mice. With inhalation anesthesia, the heart rate (HR) and SPO₂ were within an acceptable range. In contrast, the HR significantly decreased after initiation of pentobarbital anesthesia, and gradually returned to a low rate. Importantly, pentobarbital anesthesia significantly lowered SPO₂, and heart rate variability analysis showed unstable beat-to-beat intervals during pentobarbital anesthesia, suggesting that inhalation anesthesia is more suitable for evaluation of cardiorespiratory responses than pentobarbital anesthesia. During anesthesia, propranolol, a β-adrenergic blocker, significantly decreased heart rate. Atropine, a parasympathetic blocker, also significantly increased heart rate. Our data suggest that inhalation anesthesia is suitable for cardiorespiratory analysis in mice.
収録刊行物
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- 弘前医学
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弘前医学 67 (1), 77-85, 2016
弘前大学大学院医学研究科・弘前医学会
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詳細情報 詳細情報について
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- CRID
- 1390569612375697152
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- NII論文ID
- 130008041764
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- NII書誌ID
- AN00211444
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- ISSN
- 24344656
- 04391721
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- HANDLE
- 10129/5923
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- NDL書誌ID
- 027600955
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可