Noise Elimination Electrical Impedance Spectroscopy (neEIS) System for Single Cell Identification
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- TRAN A.Kiet
- Chiba University
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- KAWASHIMA Daisuke
- Chiba University
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- SUGARAWA Michiko
- Chiba University
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- OBARA Hiromichi
- Tokyo Metropolitan University
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- OKEYO Kennedy Omondi
- Kyoto University
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- TAKEI Masahiro
- Chiba University
Abstract
<p>We propose a noise elimination electrical impedance spectroscopy (neEIS) system for single cell identification whose characteristics are cell-free clamp structure and cell-position independent calibration. The cell-position independent calibration consists of three steps; impedance measurement, electrode cell constant calculation, and single cell dielectric properties estimation. The cell-position independent calibration enables elimination the influence of noise associated with ions as well as electrical double layer during the measurement process. We demonstrate an application of neEIS system to discriminate three types of MRC-5 human lung fibroblasts; wild type (WT), GFP-fused histone (HT) modification, and GFP transfected (GFPT) MRC-5 cells suspended in a sucrose medium. Taken together, these results demonstrate that the proposed neEIS system has the potential to improve the performance of biosensors. Thus, this study will bring a new insight into the development of a biosensor system for high accuracy single cell identification as well as inspire the development of a diagnostic device for early fetal lung diseases based on the neEIS system.</p>
Journal
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- The Proceedings of the JSME Conference on Frontiers in Bioengineering
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The Proceedings of the JSME Conference on Frontiers in Bioengineering 2020.31 (0), 1A14-, 2020
The Japan Society of Mechanical Engineers
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Details 詳細情報について
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- CRID
- 1390570078121737600
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- NII Article ID
- 130008062142
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- ISSN
- 24242810
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed