Intrauterine Repair of Cleft Lip in Mouse Fetuses

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  • Intrauterine repair of cleft lip in mou

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ABSTRACT Since most fetuses with drug-induced cleft lip have concurrent cleft pa- late, they die shortly after birth as a result of difficulty in suckling. We devised an ex- perimental model, in which cleft lip unaccompanied by cleft palate was produced artificially, in order to permit long-term follow-up of intrauterine cleft lip repair surgery. Fifteen Jc1:ICR mice each on days 15, 16, 17 and 18 of gestation (the day when a vaginal plug was found = day 0 of gestation) were used. Each mouse was laparotomized by midline incision of the lower abdomen, and the fetus at the distal end or the fetus adjacent to the fetus at the distal end of the uterine horn was subjected to surgery. Using a surgical microscope, the uterine wall was incised, and the amnion and chorion were pierced to expose the snout of the fetus. After unilateral complete cleft lip was created, the wound was repaired with 10-0 nylon sutures. The snout was then replaced within the uterus, and the surgical wound in the uterine wall was closed. The uterine horn was then placed back into the peritoneal cavity, and the abdominal wound was closed. The dams were then returned to their individual cages, and the fetuses were delivered spontaneously. The live birth rate for surgically treated fetuses was 60% in animals treated on day 15 of gestation, 73% among those treated on day 16, 80% among those treated on day 17, and 60% among those treated on day 18. The offspring underwent the normal course of postnatal growth, with good jaw development and no cicatrization at the site of up- per lip surgery either grossly or histologically.

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