Development of Rabbit Whole Embryo Culture during Organogenesis
書誌事項
- タイトル別名
-
- Development of rabbit whole embryo cult
この論文をさがす
抄録
ABSTRACT The rodent embryo culture has widely been spread as a technique in teratogenicity studies. However, the whole embryo culture of rabbits has not been developed. Pregnant rabbits are usually used in a teratogenicity test as a non-rodent animal to examine the species specificity. Rabbit embryos of the Japanese White strain on day 10 of gestation (the time of copulation = day 0) were cultured at 38°C for 48 hours in 100% rabbit serum. The 35 ml bottles with 5 ml serum were gassed with 95070 02 and 5% C02 continuously. The yolk sac and amniotic sac were opened 24 hours after the beginning of the culture. At the end of the culture, embryos were examined for somite number, as well as protein content and crown-rump length. Embryos cultured for 48 hours from day 10 seemed to be well developed as well as in vivo embryos. These results showed that rabbit embryo culture became a useful system for the study of reproductive and developmental toxicology, teratology and developmental biology.
収録刊行物
-
- 日本先天異常学会会報
-
日本先天異常学会会報 31 (3), 153-156, 1991
日本先天異常学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390570319884069632
-
- NII論文ID
- 110002786362
- 30037124109
-
- NII書誌ID
- AN10066760
-
- ISSN
- 09143505
- 24331503
- 17414520
- 00372285
-
- NDL書誌ID
- 3749806
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可