Reconstitution of the immune system after murine allogeneic umbilical cord blood transplantation

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  • Sato Hideaki
    Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences
  • Sato Masashi
    Department of Medical Technology, Hirosaki University School of Health Sciences
  • Chiba Makie
    Department of Medical Technology, Hirosaki University School of Health Sciences
  • Ito Kyoko
    Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences
  • Ito Koichi
    Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences Research Center for Biomedical Sciences, Hirosaki University Graduate School of Health Sciences

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Other Title
  • マウス同種異系臍帯血移植による免疫系の再構築

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Abstract

    The ability of murine allogeneic umbilical cord blood cells (UCBCs) to reconstitute the immune system was investigated. UCBCs obtained from fetuses of C57BL/6 (B6; H-2b) mice, which were transgenic for green fluorescent protein (GFP), were transplanted into RAG2 (-/-) BALB/c mice (H-2d). After transplantation, flow cytometric analysis revealed successful reconstitution of phenotypically mature GFP-positive immune cells of donor origin, including T cells, B cells, monocytes, and granulocytes in the peripheral blood of the recipient mice. Analysis of functional maturation of lymphocytes revealed that 2,4,6-trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH)-immunized UCBC-transplanted recipients produced both TNP-specific IgM and IgG antibodies. These results indicated that the recipient mice were capable of mounting antibody responses to T-dependent antigens; further, Ig class switching from IgM to IgG confirmed that both B cells and CD4⁺ helper T cells derived from allogeneic UCBCs were immunologically competent. Furthermore, mice transplanted with allogeneic UCBCs accepted skin grafts from both B6 and BALB/c mice. However, these chimeric mice completely rejected skin grafts from third party C3H/HeJ (H-2k) mice, indicating the presence of functional CD8⁺ killer T cells as well as CD4⁺ helper T cells. In terms of potential clinical application, our results indicate that allogeneic UCBC transplantation can enable recovery of the normal immune system in recipients.

Journal

  • Hirosaki Medical Journal

    Hirosaki Medical Journal 61 (1), 35-45, 2010

    Hirosaki University Graduate School of Medicine,Hirosaki Medical Society

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