The Influence of the Heat-not-burn Cigarettes on Alveolus and Cytokine

DOI
  • MARUOKA Hiroshi
    School of Health and Social Services, Saitama Prefectural University
  • TANAKA Ken-ichi
    School of Health and Social Services, Saitama Prefectural University
  • TAKAYANAGI Masaaki
    School of Health and Social Services, Saitama Prefectural University
  • ZENDA Masashi
    School of Health and Social Services, Saitama Prefectural University Division of Rehabilitation, International University of Health and Welfare Ichikawa Hospital

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タイトル別名
  • ─The Analysis of Chronic Obstructive Pulmonary Disease (COPD) and Skeletal Muscle Atrophy Model Mice─

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<p>　Objectives: Long-term exposure to heat-not-burn cigarette smoke can serve as a risk factor for smoking-related diseases such as chronic obstructive pulmonary disease (COPD), similar to the exposure to conventional cigarettes. In this study, we evaluated the effects of heat-not-burn cigarettes on the alveoli and the cytokine expression in COPD muscle atrophy model mice treated with heat-not-burn cigarettes.</p><p>　Methods: We used 17 wild-type mice (C57BL6, male, 12 weeks old) and divided them into heat-not-burn cigarette and control groups; next, we randomly divided them into three groups depending on whether they were subjected/not subjected to hind limb suspension. The COPD muscle atrophy mice model was developed through the intratracheal administration of a solution of heat-not-burn cigarettes along with hind limb suspension of mice. The mRNA expression levels were analyzed by real-time PCR, the morphological analysis of the histological image was obtained from general staining, the macrophage expression level was examined using immunostaining, and pulmonary function was measured using spirometry.</p><p>　Results: The results indicated increases in the mean linear intercept, inflammatory cytokine expression, and macrophage count in the lungs in the COPD muscle atrophy model treated with heat-not-burn cigarettes. Besides, a decrease in the mRNA levels of optic atrophy 1 was observed during mitochondrial fusion in the muscle tissues.</p><p>　Conclusions: These results indicate that the heat-not-burn model can be used as a COPD model, and that the use of heat-not-burn cigarettes affects pulmonary tissues, inflammatory cytokine expression, macrophage count, and muscle mitochondrial dynamics in the lung.</p>

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