Evaluation of controlled release from doxorubicin-loaded nanoparticles under ultrasound irradiation

DOI
  • Shinzato Miryu
    Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Japan
  • Kato Yuji
    Faculty of Information Science and Technology, Hokkaido University, Sapporo, Japan
  • Hashimoto Mamoru
    Faculty of Information Science and Technology, Hokkaido University, Sapporo, Japan
  • Kudo Nobuki
    Faculty of Information Science and Technology, Hokkaido University, Sapporo, Japan

Bibliographic Information

Other Title
  • 超音波照射下におけるドキソルビシン付着型アルブミンナノ粒子の徐放性の評価

Search this article

Description

<p>To reduce anticancer drug side effects, doxorubicin (DOX) loaded albumin nanoparticles (DOX-Alb) that can realize targeting function to cancerous tissues and controlled DOX release by ultrasound irradiation were developed. Although DOX dynamics in the cytoplasm can be visualized by fluorescence microscopy, it is difficult to quantify the amount of DOX in the nucleus because DOX fluorescence is quenched when it intercalates with nucleic acids. A method has been proposed to evaluate DOX concentration in the nucleus by staining with Hoechst 33342 that also intercalates with nucleic acids. In this study, we applied the method to investigate the possibility of controlling DOX release by ultrasound irradiation. Experimental results indicated that cytoplasmic Dox fluorescence is higher in cells cultured with Dox-Alb and that nucleic Hoechst fluorescence is lower in cells cultured with a higher Dox concentration medium, whereas cells cultured in DOX-Alb (US+, US-) culture mediums showed the different trends.</p>

Journal

Details 詳細情報について

  • CRID
    1390571240018100736
  • NII Article ID
    130008105239
  • DOI
    10.11239/jsmbe.annual59.322
  • ISSN
    18814379
    1347443X
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

Report a problem

Back to top