Evaluation of five media for detection of <i>Staphylococcus aureus</i> and seven media for detection of methicillin-resistant <i>S. aureus</i>

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  • TO Hiromi
    Department of Clinical Laboratory, Fukuoka University Hospital
  • TOKUSHIGE Chiemi
    Department of Clinical Laboratory, Fukuoka University Hospital
  • ERA Fumiyoshi
    Department of Clinical Laboratory, Fukuoka University Hospital
  • HIGUCHI Naoko
    Department of Clinical Laboratory, Fukuoka University Hospital
  • YUKI Makiko
    Department of Clinical Laboratory, Fukuoka University Hospital
  • KAJIHARA Nozomi
    Department of Clinical Laboratory, Fukuoka University Hospital
  • OBA Chinami
    Department of Clinical Laboratory, Fukuoka University Hospital
  • SHIMADA Hirofumi
    Department of Clinical Laboratory, Fukuoka University Hospital

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Other Title
  • 5種類のブドウ球菌選択培地および7種類のMRSAスクリーニング培地の検出性能比較
  • 5シュルイ ノ ブドウ キュウキン センタクバイチ オヨビ 7シュルイ ノ MRSA スクリーニングバイチ ノ ケンシュツ セイノウ ヒカク

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Abstract

<p>To efficiently isolate methicillin-resistant Staphylococcus aureus (MRSA) using culture media, we evaluated the performance of five media for the detection of Staphylococcus aureus, and we compared the performance of seven media for the detection of MRSA with that of the drug susceptibility test. Sixty Staphylococcus strains (28 MRSA strains, 17 methicillin-susceptible Staphylococcus aureus (MSSA) strains, and 15 non-S. aureus strains) were used for evaluation of culture media. As a result, the sensitivity and specificity of culture media for the detection of S. aureus were 91%–100% and 87%–100%, respectively. Moreover, in comparison with the drug susceptibility test, the positive and negative predictive values for MRSA were 90%–100% and 94%–100%, respectively. We recommend that the properties of media used to efficiently isolate MRSA should be clarified in future studies.</p>

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