STUDY ON AN ENVIRONMENTAL DNA ANALYSIS PROTOCOL FOR SEAGRASS BED MONITORING
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- AKATSUKA Maiko
- 大成建設株式会社 技術センター
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- TAKAYAMA Yuriko
- 大成建設株式会社 技術センター
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- MUCHEBVE Edwin
- 大成建設株式会社 技術センター
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- ITO Kazunori
- 大成建設株式会社 技術センター
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- WATANABE Kenta
- 港湾空港技術研究所 沿岸環境研究グループ
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- KUWAE Tomohiro
- 港湾空港技術研究所 沿岸環境研究グループ
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- MINAMOTO Toshifumi
- 神戸大学大学院 人間発達環境学研究科
Bibliographic Information
- Other Title
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- 藻場モニタリングのための環境DNA分析プロトコル作成に向けた検討
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Abstract
<p> The utilization of environmental DNA(eDNA) is being considered as a new method for monitoring seagrass beds. In field surveys, the amount of eDNA of seagrass was below the minimum limit of quantification by using the conventional method that is often performed in freshwater areas for animals. In this study, we assumed water sample condition with a large amount of suspended solids, which is inevitable for monitoring seagrass beds using eDNA, and organize the requirements for protocolizing the analysis method for collecting a sufficient amount of DNA. We found the relationship between the seasonal change of Zostera japonica and that of the amount of eDNA by using the new analysis method for low-concentration samples. The numerical simulation indicated that suitable time for sampling was different depending on sampling sites. Additionally, these results showed the possibility of applying the eDNA analysis and numerical simulation methods for future field surveys.</p>
Journal
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- Journal of Japan Society of Civil Engineers, Ser. B2 (Coastal Engineering)
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Journal of Japan Society of Civil Engineers, Ser. B2 (Coastal Engineering) 77 (2), I_895-I_900, 2021
Japan Society of Civil Engineers
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Details 詳細情報について
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- CRID
- 1390571415709406208
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- NII Article ID
- 130008113421
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- NII Book ID
- AA12508551
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- ISSN
- 18838944
- 18842399
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- HANDLE
- 20.500.14094/90009169
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed