RNA Virosphere in a Marine Zooplankton Community in the Subtropical Western North Pacific

  • Hirai Junya
    Atmosphere and Ocean Research Institute, The University of Tokyo
  • Urayama Syun-ichi
    Laboratory of Fungal Interaction and Molecular Biology (donated by IFO), Department of Life and Environmental Sciences, University of Tsukuba Microbiology Research Center for Sustainability (MiCS), University of Tsukuba Research Center for Bioscience and Nanoscience (CeBN), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
  • Takaki Yoshiro
    Super-cuttingedge Grand and Advanced Research (SUGAR) Program, JAMSTEC
  • Hirai Miho
    Super-cuttingedge Grand and Advanced Research (SUGAR) Program, JAMSTEC
  • Nagasaki Keizo
    Faculty of Science and Technology, Kochi University
  • Nunoura Takuro
    Research Center for Bioscience and Nanoscience (CeBN), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

Search this article

Description

<p>Zooplankton and viruses play a key role in marine ecosystems; however, their interactions have not been examined in detail. In the present study, the diversity of viruses associated with zooplankton collected using a plankton net (mesh size: 100‍ ‍μm) in the subtropical western North Pacific was investigated by fragmented and primer ligated dsRNA sequencing. We obtained 21 and 168 operational taxonomic units (OTUs) of ssRNA and dsRNA viruses, respectively, containing RNA-dependent RNA polymerase (RdRp). These OTUs presented average amino acid similarities of 43.5 and 44.0% to the RdRp genes of known viruses in ssRNA viruses and dsRNA viruses, respectively. Dominant OTUs mainly belonged to narna-like and picorna-like ssRNA viruses and chryso-like, partiti-like, picobirna-like, reo-like, and toti-like dsRNA viruses. Phylogenetic ana­lyses of the RdRp gene revealed that OTUs were phylogenetically diverse and clustered into distinct clades from known viral groups. The community structure of the same zooplankton sample was investigated using small subunit (SSU) rRNA sequences assembled from the metatranscriptome of single-stranded RNA. More than 90% of the sequence reads were derived from metazoan zooplankton; copepods comprised approximately 70% of the sequence reads. Although this ana­lysis provided no direct evidence of the host species of RNA viruses, these dominant zooplankton are expected to be associated with the RNA viruses detected in the present study. The present results indicate that zooplankton function as a reservoir of diverse RNA viruses and suggest that investigations of zooplankton viruses will provide a more detailed understanding of the role of viruses in marine ecosystems.</p>

Journal

  • Microbes and Environments

    Microbes and Environments 37 (5), n/a-, 2022

    Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Citations (1)*help

See more

References(59)*help

See more

Related Projects

See more

Details 詳細情報について

Report a problem

Back to top