PROPERTIES OF THE ISOLATED CORTEX OF THE MEDAKA EGG

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Abstract

The release of cortical alveoli (CA) from the isolated egg cortex of the medaka, Oryzias latipes, was observed at the threshold level of 1 mM Ca^<2+> at pH 6.7, which on addition of phospholipase A_2 (PLase A_2) was lowered to 50 μM Ca^<2+>. The addition of ATP at 1 mM further lowered the threshold level to 1 μM Ca^<2+>. When the cortex was mechanically removed in a Ca-free medium, many protrusions were observed on the surface of the intact CA. The process of CA release in the isolated cortex was proposed.

Journal

  • Medaka

    Medaka 3 17-23, 1985-12

    Laboratory of Freshwater Fish Stocks, Nagoya University

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