Inorganic contents of the medaka egg before and after cortical reaction

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Abstract

Major anionic and cationic contents of medaka Oryzias latipes eggs were measured by ion chromatography and atomic absorption spectrophotometry, respectively. Differences in the total amounts of respective inorganic ions as determined with the ashes of eggs were hardly recognizable before and shortly after exocytosis. However, a decrease in the content of free Ca^<2+> and Mg^<2+> was recognized in dialysates of egg homogenates shortly after the initiation of exocytosis. The present data for O. latipes eggs suggest that changes in these cationic contents may be associated with the process of egg activation.

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