Spatiotemporal pattern of microtubules in parthenogenetically activated Oryzias latipes (medaka) eggs

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The overall pattern of ooplasmic segregation, the movement of the female pronucleus, and the spatiotemporal pattern of microtubules were all very similar in parthenogenetically activated vs. fertilized medaka eggs. With respect to microtubules, a monaster formed at the animal pole by T_n?0.25 (T_n, normalized time, in which the time between activation and the first cleavage of a fertilized eggs is represented by one unit), persisted until T_n?0.75, and began to disintegrate at T_n?0.8-0.9, when fertilized eggs entered mitosis. Interpolar ooplasm contained a network of microtubules, most of which showed no apparent preferred orientation. The density of this network decreased at T_n?0.75-1.0, especially near the animal pole. At the vegetal pole of the egg, an array of parallel microtubules began to form at T_n?0.25. This array was the dominant feature of the vegetal pole region at T_n?0.45, but by T_n?0.75 it had begun to disintegrate. These results show that the egg itself can assemble and disassemble complex networks of microtubules and organize the movements that constitute ooplasmic segregation in this species.

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