Effects of Palmitic Acid on the Binding of Insulin^-131I to the Rat Liver Mitochondria

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Effects of palmitic acid, inhibitors, uncouplers, and reduced and oxidized glutathione on the binding of insulin^-131I and 131I to the isolated fresh or aged mitochondria were studied. The binding of insulin^-1311 to the fresh mitochondria was unexpectedly increased to a greater degree with the exogenous addition of palmitic acid, glutathione, acetaldehyde and 2,4-dinitrophenol to the medium. The fact indicated that the intactness of mitochondria was not prerequisite for the binding of insulin^-131I to the isolated mitochondria. The binding of insulin^-131I was compared to the fresh mitochondria and the aged mitochondria. Insulin^-131[ bouud to the aged mitochondria in a lesser degree. It is suggested that the binding of insulin^-131I to the mitochondria might be related to the mitochondrial functional and conformational state. The binding of 131I to the mitochondria was apparently differrnt from that of insulin^-131I. And the binding of 131I was not altered by these uncouplers except in the case of acetaldehyde and ethyl alcohol. This implies that the process of binding of insulin^-131I is different from that of 131I. In the case of 131I, it may have some connections with its ion transport mechanism.

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