Preparation of Anti-2,4-dinitrotoluene Monoclonal Antibody by Using Rat Medial Iliac Lymph Node Cells and Its Characterization Using Solid Phase Enzyme-linked Immunosorbent Assay

DOI HANDLE 被引用文献1件 オープンアクセス
  • 長友 一剛
    九州大学大学院農学研究院生物機能科学部門食品バイオ工学
  • 松本 清
    九州大学大学院農学研究院生物機能科学部門食品バイオ工学
  • 都甲 潔
    九州大学大学院システム情報科学研究院
  • 三浦 則雄
    九州大学産学連携センター

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Recent concern on international terrorism and weapons of mass destruction demands the development of novel analytical methods for identification and quantification of explosive molecules. 2,4.Dinitrotoluene (2,4-DNT) is one of the decomposed compounds from 2,4,6-trinitrotoluene (TNT), a main explosive compound, and has high vapor pressure and life time than TNT. 2,4-DNT, thus, become a chemical signature of explosives. Monoclonal antibody against 2,4.DNT was prepared by using rat lymph node method. Four hybridoma cells which produce anti.DNPh antibody were produced out of 61 fusion positive cells. The best hybridoma cell (named DNPh-mAb2 cell) was selected for the production of monoclonal antibody (DNPh. mAb2). The properties of DNPh-mAb2 were investigated such as cross-reactivity and affinity constant. The prepared DNPh.mAb2 has high avidity to 2,4-DNT compared with other nitroaromatic compounds except N-(2,4,6-tirinitrophenyl) glycine (TNP-Gly, ca. 50%). TNP-Gly is not contained in general explosives, appearing no problem. The antibody has high affinity constant of 3.6×10^-6mol L^-1 to 2,4-DNT. The limit of detection of 2,4-DNT by indirect competitive ELISA was ca. 5 ng mL^-1, showing suitable for 2,4-DNT detection.

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詳細情報 詳細情報について

  • CRID
    1390572174638292096
  • NII論文ID
    120001204032
  • NII書誌ID
    AA00247166
  • DOI
    10.5109/14055
  • HANDLE
    2324/14055
  • ISSN
    00236152
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • IRDB
    • Crossref
    • CiNii Articles

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