ネパール原産のイネ品種「Pakhe Dhan」が持つ障害型耐冷性 QTL (qFLT-6) 遺伝子の「ほしのゆめ」への導入効果の検証

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  • Effect of introgression of a QTL (qFLT-6) gene for cold tolerance at the booting stage derived from a Nepalese rice cultivar, "Pakhe Dhan" to "Hoshinoyume".
  • ネパール ゲンサン ノ イネ ヒンシュ Pakhe Dhan ガ モツ ショウガイガタ タイレイセイ QTL qFLT 6 イデンシ ノ ホシ ノ ユメ エノ ドウニュウ コウカ ノ ケンショウ

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説明

qFLT-6 is a quantitative trait locus (QTL) for cold tolerance at the booting stage that was identified by using a Nepalese cold-tolerant rice cultivar, "Pakhe Dhan". Here, we report the effect of the "Pakhe Dhan" allele of qFLT-6 on a Hokkaido rice cultivar, "Hoshinoyume". We evaluated cold tolerance of F_2, BC_1F_2 and BC_2F_2 populations between a donor parent, "Fukei-212" or "04PM254", and a recurrent parent, "Hoshinoyume" by the cool-water irrigation method. We also genotyped each plant using an SSR marker, FP0612, located near qFLT-6 . Cold tolerance of the plants with the "Pakhe Dhan" allele was significantly higher than that of the plants with the "Hoshinoyume" allele in certain BC_1F_2 and BC_2F_2 populations with early heading, but there was no clear effect of introgression of the "Pakhe Dhan" allele of qFLT-6 on "Hoshinoyume". On the other hand, there was no significant difference in heading date and culm length between marker classes. The results suggested that introgression of the "Pakhe Dhan" allele of qFLT-6 was effective for increasing cold tolerance of "Hoshinoyume" under some genetic background and that there was no linkage drag of unfavorable traits such as late heading and long culm.

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