Evaluation of Leaf Photosynthetic Potential Based on Chlorophyll Fluorescence from a Vaseline-sealed Leaf in Sweet Potato

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  • ワセリン・シーリング葉のクロロフィル蛍光値によるサツマイモ個葉の炭酸固定能力の評価
  • ワセリン シーリング ハ ノ クロロフィル ケイコウチ ニ ヨル サツマイモコ ヨウ ノ タンサン コテイ ノウリョク ノ ヒョウカ

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Abstract

The leaf photosynthetic potential was evaluated based on the electron transport rate (ETR) of the photosystem Ⅱ in a sweet potato leaf the surface of which was sealed with Vaseline to stop the gas exchange flow. Directly after the total carbon assimilation rate (Pg_2%, or photosynthetic potential) of a leaf was measured in 2%-O_2 air, the leaf was sealed with Vaseline and the ETR was measured. These measurements were tested using young to aged leaves at different light intensities. A close positive relationship was found between Pg_2% and ETR. This result indicates that Pg_2%, or the leaf photosynthetic potential, is estimated by this method. When the detached leaf was Vaseline-sealed and placed in a moistened vinyl-bag in the shade at room temperature, the activity of the preserved leaves was sustained for more than two hours after sampling. Since the chlorophyll fluorescence of a Vaseline-sealed leaf can be monitored within a few minutes, a lot of stocked leaves are possible to be measured collectively. The value obtained is used as a selection criterion for the leaf photosynthetic.

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