Inquiries About Biomarkers of Acute Liver Failure in Patients Who Underwent Living Donor Liver Transplantation Using a Protein Chip Array

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  • Yamashita Yo-ichi
    Department of Hepato-Biliary-Pancreatic Surgery, National Kyushu Cancer Center | Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University | Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University
  • Yoshizumi Tomoharu
    Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University
  • Ikegami Toru
    Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University
  • Uchiyama Hideaki
    Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University
  • Tsujita Eiji
    Department of Hepato-Biliary-Pancreatic Surgery, National Kyushu Cancer Center
  • Itoh Shinji
    Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University
  • Harimoto Norifumi
    Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University
  • Soejima Yuji
    Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University
  • Taketomi Akinobu
    Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University
  • Baba Hideo
    Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University
  • Maehara Yoshihiko
    Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University

Bibliographic Information

Other Title
  • プロテインチップアレイを用いた急性肝不全生体肝移植患者におけるバイオマーカーの検討

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Abstract

The causative agent of hepatic encephalopathy (HE) has not been identified with certainty. The recovery of consciousness in patients with acute liver failure (ALF) who underwent liver transplantation (LT) is sometimes drastic ; therefore, we thought that the causative agents of HE would change markedly peri-operatively in these patients. We examined the biomarkers including new agents in the serum of patients using the ProteinChip^【○!R】 System 4000 (Ciphergen Biosystems, Yokohama, JAPAN). Sixteen samples were obtained from four patients with ALF who underwent living donor LT (LDLT) at four time points ; pre-operative, one post-operative day (1POD), 3POD, and 7POD. We used three chips made by the Biomek2000 robot. All duplicated samples were assayed and analyzed using the CiphergenExpress^<TM> data manager. We divided the peri-operative changes in the intensity of identified peaks into seven patterns. The number of peaks whose intensity shows significant changes peri-operatively reached 755. Of course, it is difficult to determine each structure in all 755 peaks ; therefore, we should narrow down the candidates for causative agents of HE in further studies. Our own results suggest that many difficulties lie ahead in determining the causative agent of HE.

Journal

  • 福岡醫學雜誌

    福岡醫學雜誌 107 (7), 131-135, 2016-07-25

    Fukuoka Medical Association

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