Analysis of the Neuraminidase Amino Acid Sequences of Influenza A/H1N1pdm09, A/H3N2, and B Viruses Isolated from Influenza Patients in the 2015/16 Japanese Influenza Season

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  • Ikematsu Hideyuki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital | Japan Physicians Association, Influenza Study group
  • Chong Yong
    Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences | Graduate School of Medical Sciences, Kyushu University : Assistant professor
  • Matsumoto Shinya
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital
  • Noda Nozomi
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital
  • Hotta Taeko
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital
  • Uchiumi Takeshi
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital | Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University
  • Kang Dongchon
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital | Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University

Bibliographic Information

Other Title
  • 2015/16年シーズンに日本で患者より分離されたインフルエンザウイルス，A/H1N1pdm09，A/H3N2，およびBのノイラミニダーゼ遺伝子と薬剤感受性との関連についての検討
  • 2015/16ネン シーズン ニ ニホン デ カンジャ ヨリ ブンリ サレタ インフルエンザウイルス,A/H1N1pdm09, A/H3N2,オヨビ B ノ ノイラミニダーゼ イデンシ ト ヤクザイ カンジュセイ ト ノ カンレン ニ ツイテ ノ ケントウ

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Abstract

Background : Neuraminidase (NA) is an essential surface protein for influenza virus replication. NA inhibitors are commonly used for the treatment of influenza patients in Japan, thus emergence of resistant viruses is of great concern. We sequenced the NA segment of isolated virus from influenza patients in the 2015-16 influenza season and investigated the relation between the deduced NA amino acid sequence and the 50% inhibitory concentration (IC_50) of four NA inhibitors. Materials and Methods : A total of 59 viruses (20 A/H1N1pdm09, 19 A/H3N2, and 20 B) that showed high or low IC_50 to NA inhibitors were sequenced using a “next generation sequencer”, and the deduced amino acid sequences were analyzed. Results : Two A/H1N1pdm09 viruses that showed very high IC_50 for oseltamivir (150 nM and 130 nM) contained the H275Y mutation that has been reported in several previous seasons. Except for this H275Y mutation, no significant relation was found between the NA amino acids and the IC_50 of the four NA inhibitors in A/H1N1pdm09 viruses. There was no significant relation between the NA amino acids and the IC_50 of the four NA inhibitors for A/H3N2. NA amino acids sequences of B were divided into two groups. There was no significant relation between the NA amino acids and the IC_50 of the four NA inhibitors for B. Conclusion : The previously reported H275Y mutation that causes oseltamivir resistance was found in two A/H1N1pdm09 viruses that showed a very high IC_50 for oseltamivir. No additional NA amino acid sequences related to the IC_50 of four NA inhibitors was found in other virus in the 2015-16 influenza season.

Journal

  • 福岡醫學雜誌

    福岡醫學雜誌 108 (7), 194-203, 2017-07-25

    Fukuoka Medical Association

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