ヒトアデノウイルス12型誘発腫瘍細胞のウイルスゲノム

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タイトル別名
  • Viral Genome in Rodent Cells Transformed by Human Adenovirus Type 12
  • ヒトアデノ ウイルス 12ガタ ユウハツ シュヨウ サイボウ ノ ウイルス ゲ

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Adenovirus type 12, Huie strain, (Ad 12) DNA was cleaved with HindIII, a restriction endonuclease from Hemophilus influenzae (Rd strain), into 16 specific fragments (HindIII-A?HindIII-O). Several lines of Ad 12-transformed rodent cells were examined for the persisting viral DNA sequences by reassociation kinetics using DNA fragments as probes. As a result it was shown that each of the cell lines examined contained the nucleotide sequence of the HindIII-G fragment. This indicates that the detection and quantitation of the transforming segment (included in HindIII-G fragment) in cell DNA is important for the investigation of Ad 12 etiology of tumors including human neoplasia.

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