多電極電位計測システムを用いた心毒性検査の培地条件探索及び、ニコチン添加によるニワトリ胚由来心筋細胞への影響

DOI HANDLE

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タイトル別名
  • EXPLORATION OF MEDIUM CONDITIONS FOR A CARDIOTOXICITY TESTING BY MULTI ELECTRODE ARRAY SYSTEM AND EFFECTS ON CHICKEN EMBRYO-DERIVED CARDIOMYOCYTES BY NICOTINE ADDITION

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Nicotine has been suggested that may directly act onCa2+ pump in sarcoplasmic reticulum and K+ channel of cardiomyocytes. To verify them, multi electrode array (MEA)system that can measure extracellular field potential was adopted. I explored the optimal medium conditions because MEA system protocols have not been established. As a result, extracellular field potential of cardiomyocytes was stably measured in DMEM with 25 mM HEPES. Therefore I used this medium for the examination of nicotine effect to cardiomyocytes. Nicotine shortened interspike interval and prolonged field potential duration. From these results, it was suggested that nicotine directly promoted the function of Ca2+ pump in sarcoplasmic reticulum and inhibited the function of K+ channel of cardiomyocytes.

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詳細情報 詳細情報について

  • CRID
    1390572174784184704
  • NII論文ID
    120005616093
  • NII書誌ID
    AA12677220
  • DOI
    10.15002/00011427
  • HANDLE
    10114/10741
  • ISSN
    21879923
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • IRDB
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用可

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