ウニ胚の脂質代謝(予報)

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タイトル別名
  • Lipid Metabolism of Sea Urchin Embryos (A Preliminary Report).

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Up to the present, there have been obtained little informations about the lipid metabolism during the early development of sea urchins. We have investigated the incorporation if acetate-1-C^<14> and of glycerol-1-C^<14> into the lipid of sea urchin embryos, using a Mediterranean species, Paracentrotus lividus. The eggs were deprived of jelly with acid sea water, fertilized and cultured in a large volume of sea water with constant stirring at 18℃. At desired stage of development, eggs and embryos were centrifuged down, suspended in a small volume of sea water containing 10-20 μc of substrate and incubated for 2 hr. After the incubation, they were sedimented again, washed with sea water and extracted with a chloroform-methanol mixture (2:1) according to Folch et al. (1957). A EKCO scintillation counter was used for measuring radioactivity. As can be seen from Fig. 1, the incorporation of C^<14>-acetate into the total lipid increased remarkably with the progress of development, reaching a maximum at the prism stage. A similar curve was obtained with C^<14>-glycerol, although the lipid was much less labeled with this substrate. Since the amount of lipid begins to decrease after the gastrula stage, accompanied with a reduction in R. Q., the above results appear to reflect at a glance the augmentation of lipid metabolism as a whole. As indicated with the dotted line in Fig. 1, however, if blastulae were incubated with C^<14>-acetate for 2 hr., washed with sea water and then cultured further in sea water containing an excess C^<12>-acetate up to the prism stage, there occurred little loss of radioactivity in the total lipid. The result seems to indicate a real new synthesis of some lipid components during the later stages of early development of the sea urchin. The details of this work will be reported elsewhere.

収録刊行物

  • 動物学雑誌

    動物学雑誌 72 (9), 265-266, 1963-09-15

    東京動物學會

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