Development of Basic Proteomics Technology Using Monolithic Silica Columns and Its Application to Analysis of Pluripotent Stem Cells
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- Iwasaki Mio
- Kyoto University, Center for iPS Cell Research and Application
Bibliographic Information
- Other Title
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- モノリスシリカカラムを用いたプロテオミクス基盤技術の開発と多能性幹細胞解析への展開
Abstract
<p>Current proteome analysis method is an effective method to comprehensively analyze the proteins, but it is still difficult to identify and quantify the proteins of a specific cell in a single analysis due to the complexity of the proteome samples. We have been working on the development of basic technologies to solve this problem and improve the identification and quantification efficiency. We have focused on the use of monolithic columns to improve separation efficiency and have developed the RiMS (removal of interference mixture spectra) method, which improves the accuracy of isobaric labelling quantification. We applied this method to the analysis of pluripotent stem cells, resulting improved quantification accuracy of cell type-specific proteins. In this paper, we will introduce the RiMS method with an overview of the methods used for quantitative proteome analysis.</p>
Journal
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- Proteome Letters
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Proteome Letters 6 (2), 61-68, 2021
Japanese Proteomics Society
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Details 詳細情報について
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- CRID
- 1390572244931745792
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- NII Article ID
- 130008140152
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- ISSN
- 24322776
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed