Irregular antibody screening using fully automatic blood transfusion test device Erytra Eflexis

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  • KATOU Chiaki
    Department of Medical Technique, Nagoya University Hospital
  • WATANABE Juri
    Department of Medical Technique, Nagoya University Hospital
  • MATSUOKA Hiroki
    Department of Medical Technique, Nagoya University Hospital
  • ENDOU Hiroko
    Department of Medical Technique, Nagoya University Hospital
  • WATANABE Tomomi
    Department of Medical Technique, Nagoya University Hospital
  • MATSUSHITA Tadashi
    Department of Transfusion, Department of Clinical Laboratory Medicine, Nagoya University Hospital

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Other Title
  • 全自動輸血検査装置Erytra Eflexisを用いた不規則抗体スクリーニング
  • ゼン ジドウ ユケツ ケンサ ソウチ Erytra Eflexis オ モチイタ フキソク コウタイ スクリーニング

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Abstract

<p>A fully automatic blood transfusion test device can prevent dispensing errors, eliminate individual differences in agglutination judgment, and minimize labor saving. We conducted a basic study of irregular antibody screening by the gel column agglutination method (Gel-CAT) using the fully automatic blood transfusion test device Erytra Eflexis®. The device was not affected by abnormal levels of colours routinely encountered in chylothorax, hemolysis, and hyperbilirubinemia. It was only affected by a high immunoglobulin level at IgG 7,371 mg/dL in the low-ionic-strength solution indirect antigulobulin test (LISS-IAT) and in specimens that produced rouleaux formation in the enzymatic method using papain. In the comparison of Gel-CAT with the glass-bead-based column agglutination method (Glass-CAT) using this device, Gel-CAT showed strong reaction intensity with anti-Kidd, anti-Duffy, and anti-Diego antibodies in LISS-IAT, and both anti-e and anti-Dia antibodies were detectable only by Gel-CAT. In the enzymatic method, Gel-CAT showed a strong reaction intensity with anti-Rh, anti-Kidd, and anti-Lewis antibodies, and three anti-Rh antibodies and two anti-Kidd antibodies were detected only by Gel-CAT. In samples with early antibody production, anti-E antibodies were detected earlier by Gel-CAT with the enzymatic method using papain. Gel-CAT detected anti-Jkb, anti-Dia, and anti-Lea antibodies similarly to PEG-IAT. Gel-CAT was particularly effective in detecting anti-Rh antibodies. For anti-D antibodies, the detection sensitivities were 0.02 IU/mL for LISS-IAT and 0.01 IU/mL for the enzymatic method using papain. Therefore, we concluded that Eflexis is useful for routine tests.</p>

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