Diagnostic utility of the basophil activation test in natto-induced hypersensitivity
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- Fukuda Risa
- Department of Dermatology, Tokyo Dental College Ichikawa General Hospital Department of Dermatology, Keio University School of Medicine
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- Ouchi Takeshi
- Department of Dermatology, Keio University School of Medicine
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- Asahina Yasuhiko
- Department of Dermatology, Keio University School of Medicine
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- Shiiya Chihiro
- Department of Dermatology, Keio University School of Medicine
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- Takeuchi Sakiko
- Department of Dermatology, Keio University School of Medicine
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- Yasuda-Sekiguchi Fumiyo
- Department of Dermatology, Tokyo Dental College Ichikawa General Hospital Department of Dermatology, Keio University School of Medicine
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- Iriki Hisato
- Department of Dermatology, Keio University School of Medicine
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- Kouno Michiyoshi
- Department of Dermatology, Tokyo Dental College Ichikawa General Hospital
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- Takahashi Shinichi
- Department of Dermatology, Tokyo Dental College Ichikawa General Hospital
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- Tanikawa Akiko
- Department of Dermatology, Keio University School of Medicine
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- Amagai Masayuki
- Department of Dermatology, Keio University School of Medicine
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- Takahashi Hayato
- Department of Dermatology, Keio University School of Medicine
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説明
<p>Background: Natto (fermented soybeans)-induced hypersensitivity is characterized by delayed symptom onset that hampers diagnosis. We aimed to clarify the clinical utility of the basophil activation test (BAT) in the diagnosis of natto-induced hypersensitivity.</p><p>Methods: Five patients with a history of anaphylaxis and chronic urticaria suspected of natto-induced hypersensitivity and seven with chronic spontaneous urticaria clinically unrelated to natto were enrolled in the patient and control groups, respectively. The BAT was performed with two incubation times, 15 min and 1 h, in combination with various concentrations of natto-mucilage extract.</p><p>Results: In controls, CD203c levels in basophils remained low in the 15-min incubation but were significantly increased in the 1-h incubation. In the patient group, in the 15-min condition, basophil CD203c was significantly upregulated by natto mucilage but not by soybean vs controls (P = 0.001). Low concentrations of natto mucilage were sufficient to upregulate basophil CD203c in the anaphylaxis cases, but high concentrations were required to induce the same effect in the urticaria cases. Finally, the dose-dependent pattern of the BAT results differed significantly between the anaphylaxis and urticaria cases (P = 0.006). Thus, a strong background reaction was observed in the BAT with 1 h incubation; 15 min of incubation was sufficient to identify patients with natto-induced hypersensitivity and may distinguish the clinical phenotype of natto-induced hypersensitivity, i.e., anaphylaxis or urticaria.</p><p>Conclusions: The BAT with a 15-min incubation period is useful in diagnosing natto-induced hypersensitivity.</p>
収録刊行物
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- Allergology International
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Allergology International 71 (1), 125-130, 2022
一般社団法人日本アレルギー学会
