Study of Yeast Identification Using MALDI-TOF MS

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  • Baba Hiroshi
    Japan Food Research Laboratories Saito Laboratory
  • Matsumoto Takuro
    Department of Life Science, Faculty of Life Science, Kyusyu Sangyo University
  • Matsushita Kaori
    Department of Life Science, Faculty of Life Science, Kyusyu Sangyo University
  • Nakayama Motokazu
    Department of Life Science, Faculty of Life Science, Kyusyu Sangyo University
  • Miyamoto Takahisa
    Department of Bioscience & Biotechnology, Faculty Agriculture, Kyusyu University

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Other Title
  • MALDI-TOF MSを用いた酵母の同定の検討
  • MALDI-TOF MS オ モチイタ コウボ ノ ドウテイ ノ ケントウ

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Abstract

<p>After culturing 75 strains of 19 yeast species from food on potato dextrose agar medium at 25 °C for 5 days, mass spectra were acquired using VITEK MSTM plus to prepare SuperSpectra (SSp). Verification of the prepared SSp showed that the reliability of identification was more than 80 % except for Schizosaccharomyces spp., Sterigmatomyces elviae, Starmerella apicola, S. etchellsii, Wickerhamomyces subpelliculosus, Zygosaccharomyces rouxii, and Z. bisporus. It was difficult to distinguish Z. bailii, Z. parabailii, Z. pseudobailii, Z. mellis, and Z. siamensis, by using their SSp, but they can be identified as Z. bailii complex for Z. bailii, Z. parabailii and Z. pseudobailii, and Z. mellis-siamensis complex for Z. mellis and Z. siamensis. The identification reliability level was affected by the medium used for cultivation of yeast. For the Brettanomyces (Dekkera) spp., there were cases where they were difficult to identify when a mass spectrum with peaks that had a sufficient number and intensity was not obtained, or when the major peaks were similar. Improvement of the methods for sample treatment and SSp creation were required.</p>

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