Polymethyl methacrylate resin for provisional restoration affects rat macrophage function in <i>in vitro</i> conditions
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- Đorđević Nadica S.
- Department of Dentistry, Faculty of Medicine, University of Priština in Kosovska Mitrovica
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- Šehalić Meliha G.
- Department of Dentistry, Faculty of Medicine, University of Priština in Kosovska Mitrovica
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- Tričković-Vukić Dragana
- Institute for biomedical research, Faculty of Medicine, University of Niš
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- Lazić Dragoslav D.
- Department of Dentistry, Faculty of Medicine, University of Priština in Kosovska Mitrovica
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- Marjanović Dragan D.
- Department of Dentistry, Faculty of Medicine, University of Priština in Kosovska Mitrovica
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- Radosavljević Radivoje D.
- Department of Dentistry, Faculty of Medicine, University of Priština in Kosovska Mitrovica
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- Todić Jelena T.
- Department of Dentistry, Faculty of Medicine, University of Priština in Kosovska Mitrovica
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- Tabaković Saša Z.
- Department of Dentistry, Faculty of Medicine, University of Priština in Kosovska Mitrovica
書誌事項
- 公開日
- 2022
- 資源種別
- journal article
- DOI
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- 10.2334/josnusd.22-0016
- 公開者
- 日本大学歯学部
この論文をさがす
説明
<p>Purpose: This study evaluated the cytotoxic effects of polymethyl methacrylate resin extracts on rat macrophage viability in in vitro conditions.</p><p>Methods: Prepared test specimens were immersed in 5 mL of artificial saliva and incubated for 24, 48, and 72 h at 37°C. The cytotoxicity of the obtained solutions of extracted resins, used as a stock solution (100%) and diluted with Roswell Park Memorial Institute (RPMI) medium to obtain the working solutions (50, 40, 30, 20, 10, and 5%), was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.</p><p>Results: No dose-dependent toxic activity in macrophage culture was detected for the three types of extracts obtained after 24, 48, and 72 h of material extraction. The shortest extraction of material was found to be completely non-toxic, and the 20% concentration of this extract obtained caused a significant increase in cell ability to metabolize MTT. Extracts obtained after 72 h of extraction showed the highest cytotoxic potential of 50%, 40% and 30%, and extracts obtained after 48 and 72 h of extraction at concentrations of 5% and 10% had a proliferative effect on the macrophage cell line.</p><p>Conclusion: This study demonstrated that the highest cytotoxic effect was observed in cells exposed to the highest concentrations (50, 40, and 30%) of the extracts that were extracted for 72 h.</p>
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ
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Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ 64 (3), 228-231, 2022
日本大学歯学部
