The potential of organoids in toxicologic pathology: Histopathological and immunohistochemical evaluation of a mouse normal tissue-derived organoid-based carcinogenesis model
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- Ishigamori Rikako
- Central Animal Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 Japan
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- Naruse Mie
- Central Animal Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 Japan
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- Hirata Akihiro
- Laboratory of Veterinary Pathology, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
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- Maru Yoshiaki
- Department of Molecular Carcinogenesis, Chiba Cancer Center Research Institute, 666-2 Nitonacho, Chuo-ku, Chiba 260-8717, Japan
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- Hippo Yoshitaka
- Department of Molecular Carcinogenesis, Chiba Cancer Center Research Institute, 666-2 Nitonacho, Chuo-ku, Chiba 260-8717, Japan
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- Imai Toshio
- Central Animal Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 Japan Department of Cancer Model Development, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 Japan
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抄録
<p> Recently, we introduced an organoid-based chemical carcinogenesis model using mouse normal tissue-derived organoids. In the present review article, the histopathological and immunohistochemical characteristics of mouse normal tissue-derived organoids and tumors derived from these organoids after their in vitro treatment with genotoxic carcinogens and injection into nude mouse are reviewed. In organoids treated in vitro with genotoxic carcinogens, we confirmed macroscopic tumorigenicity and histopathological findings, including neoplastic characteristics, such as multilayered epithelia and/or invasion of epithelia into the surrounding interstitium. In contrast glandular/cystic structures with monolayered epithelia were clearly demarcated from the surrounding Matrigel/interstitium in the untreated control groups. In addition to macroscopic tumorigenicity, these microscopic epithelial changes, which are characteristic of the early stages of carcinogenesis, are included in the requirements for carcinogenicity-positive judgement of the organoid-based carcinogenesis model. Immunohistochemistry of cytokeratins (CKs), used to determine the origin of epithelia and distribution of extraductal invasive lesions, or oncogenic kinases, which reflect molecular activation in epithelia following chemical treatment, is helpful for accurate diagnosis and molecular evaluation in the early stages of carcinogenesis. This information improves our biological understanding of organoid-based chemical carcinogenesis models.</p>
収録刊行物
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- Journal of Toxicologic Pathology
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Journal of Toxicologic Pathology 35 (3), 211-223, 2022
日本毒性病理学会
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詳細情報 詳細情報について
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- CRID
- 1390574181078691456
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- NII書誌ID
- AN10232280
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- ISSN
- 1881915X
- 09149198
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- NDL書誌ID
- 032294641
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可