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Development of the Practical Method for Ion Influx, Efflux, and Net Flux Measurement in Plant Roots
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- Kobayashi Natsuko I.
- Graduate School of Agricultural and Life Sciences, the University of Tokyo
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- Yang Xiaoyu
- Graduate School of Agricultural and Life Sciences, the University of Tokyo
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- Iwata Ren
- Cyclotron and Radioisotope Center, Tohoku University
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- Suzuki Hisashi
- National Institute for Quantum Science and Technology
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- Nakanishi Tomoko M.
- Graduate School of Agricultural and Life Sciences, the University of Tokyo Hoshi University
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- Tanoi Keitaro
- Graduate School of Agricultural and Life Sciences, the University of Tokyo
Bibliographic Information
- Other Title
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- 植物の根におけるイオンの流入，流出，正味流量の測定法の確立
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Description
<p>Tracer experiments using radioisotope are powerful for the analysis of mineral uptake mechanisms in plant roots. In this study, we aimed to establish a quantitative method for measuring ion fluxes, i.e. net flux, influx, and efflux, which can be used to phenotyping of the mutants and natural variations. Consistency of obtained flux values for Mg2+ and Na+ was examined, and it was found that radioisotope absorption time of 20 s gave influx rate and an absorption time of more than 15 min gave net flux rate in rice roots. Furthermore, it was suggested that the longest possible radioisotope labeling time is necessary to increase the accuracy of the calculated efflux rate.</p>
Journal
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- RADIOISOTOPES
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RADIOISOTOPES 71 (3), 179-184, 2022-11-15
Japan Radioisotope Association
